ديكسبانتينول ومستخلص صمغ النحل في تركيبة مع المضادات الحيوية المحلية لعلاج التهابات المكورات العنقودية و بسيودومونال الجرحDexpanthenol and propolis extract in combination with local antibiotics for treatment of Staphylococcal and Pseudomonal wound infections.

ديكسبانتينول ومستخلص صمغ النحل في تركيبة مع المضادات الحيوية المحلية لعلاج التهابات المكورات العنقودية و بسيودومونال الجرح.

Dexpanthenol and propolis extract in
combination with local antibiotics for
treatment of Staphylococcal and Pseudomonal
wound infections.

Ghada F. Helaly, Ahmed A. Abd El-Aziz, Fatma I.
Sonbol, Tarek E. El-Banna, Neveen L. Louisedexpanthenol-and-propolis-extract-in-combination-with-local-antibiotics-for-treatment-of-staphylococcal-and-pseudomonal-wound-infections

إدارة البكتيريا والأشكال الحيوية في الجروح أمر حيوي في تحقيق
التئام الجروح. تحديد النشاط المضاد للميكروبات والمضادات الحيوية من مختلف
العلاجات مثل ديكسبانتينول و دنج-النحل الطبيعي المنتج وحده و / أو في
جنبا إلى جنب مع العوامل المضادة للميكروبات الموضعية على الزائفة والمكورات العنقودية
وكان العدوى الجرح الهدف من هذه الدراسة

Background: Managing bacteria and their biofilms in wounds is vital in achieving
wound healing. Determination of antimicrobial and antibiofilm activity of different
remedies such as dexpanthenol and propolis- natural bee product- alone and/or in
combination with topical antimicrobial agents on Pseudomonas and Staphylococcus
wound infection was the aim of this study.
Methods and Findings: Following up on our previous study, six Staphylococcus
and Pseudomonas clinical strains selected from 80 strains isolated from wound swab
cultures through 2007-2008 were included in this study. Selection was made according
to their biofilm forming capacity (strong biofilm forming single isolates) and the
magnitude of change in their biofilm production (for isolate mixtures). The effect
of dexpanthenol on the minimum inhibitory concentrations (MICs) of four selected
topical antimicrobial agents was determined by broth micro-dilution technique using
checkerboard method as well as its antibiofilm activity. Dexpanthenol showed a
great effect on the MIC values of the tested antibiotics, as well, demonstrating high
antibiofilm activity. Dexpanthenol showed mild efflux inhibition property upon testing
with serial dilutions of ethidium bromide. The effect of ethanolic extract of propolis
(EEP) on activity of different antimicrobial agents was determined. The outcome
differs between tested isolates. Estimation of the antibiofilm activity of EEP by modified
microtiter plate method revealed a great decrease in adhesion at low concentrations
of EEP. In vivo study of the effect of selected dexpanthenol-drug combinations
on induced wound infection in rats, revealed complete healing with resolution of
infection, as evaluated histopathologically, in a shorter time.
Conclusions: Dexpanthenol and propolis alone or in combination with topically
applied antibiotics were proved effective in prevention of biofilm formation, thus
favoring wound healing in a short time.