آثار الهلام الملكي على الجلد العقيمة قطع إصلاحEffects of royal jelly on sterile skin cut repair

آثار الهلام الملكي على الجلد العقيمة قطع إصلاح

Effects of royal jelly on sterile skin cut repair
Hedayatollah Shirzad,

Effects of royal jelly on sterile skin cut repair

مقدمة: بعد الإصابة، تحدث استجابة التهابية والخلايا تحت الأدمة
تبدأ في زيادة إنتاج الكولاجين، ثم، يتم تجديد الأنسجة الظهارية. الهلام الملكي (ري)
لديه نشاط مضاد للالتهابات وبالتالي، كان الهدف من هذه الدراسة لدراسة تأثير ري على
تحريض شق الجلد العقيمة في البلب / C الفئران.
الطريقة: في دراسة تجريبية 60 أنثى البلب / C الفئران (8 أسابيع من العمر) كانت تخدير
مع الأثير وطولي الفقرة الفقري شق سماكة كاملة من 10 ملم وقدم طويلا. ال
تم تقسيم الحيوانات إلى ست مجموعات متساوية. واعتبرت المجموعة 1 سيطرة سلبية. المجموعة 2
(السيطرة الإيجابية) موضعيا مع مرهم نيتروفورازون، المجموعة 3 مع ري (200 مغ / كغ)
كل يوم، مجموعة 4 مع ري (200 ملغ / كلغ) كل يومين، المجموعة 5 مع ري (300 ملغ / كلغ) كل يوم و
مجموعة 6 مع ري (300 ملغ / كلغ) كل يومين. تم قياس طول الجرح مع كابيلار رنيه
كل يومين حتى الشفاء الكامل حدث ومقارنتها في مجموعات مختلفة.
النتائج: كان هناك فرق كبير بين المجموعات 1 أو 2 والمجموعات الأخرى (p <0.05). RJ
تعزيز نشاط التئام الجروح بشكل ملحوظ في المجموعة 3، 5 مقارنة مع السلبية والإيجابية
مجموعات السيطرة. لم يكن هناك فرق كبير بين استخدامات 200 ملغم / كغم و 300 ملغم /
كغ ري (p> 0.05).
الاستنتاج: تشير نتائج هذه الدراسة إلى أن التطبيق اليومي ل ري يمتلك الجرح بيتس
آثار الشفاء من نيتروفورازون وكل يومين استخدام ري.

Introduction
Skin is the body’s most extensive organ and is considered as a main physical barrier between a creature and its surrounding. Human’s skin protects his body against numerous harmful agents (1).
The wounds of skin are categorized into two groups, acute and chronic. Surgical wounds could be healed through different ways (2). The primary healing occurs when the tissue is incised with no infection and clean, non-infectious edges of scalpel only destroy the center of basement membrane integrity and kill a small number of epithelial and connective tissue cells. In these wounds, cut edges are close to each other and healing is complication-free. The wounds with delayed healing, has a lower tensile strength initially, but the consistency and strength become similar to normally healed wounds’ ultimately (1,2).
The stages of wound healing comprise of hemostasis and inflammation, proliferation, differentiation and reformation. Hemostasis occurs before initiation of wound inflammation. Macrophages cause regulation of cell proliferation, matrix formation, and angiogenesis through releasing of mediators such as transforming growth factor beta, vascular endothelial growth factor, insulin-like growth factor, epithelial growth factor, and lactate. In addition, macrophages contribute greatly to regulating angiogenesis as well as matrix deposition and
Maryam Shirzad1, Mahboubeh Yousofi2, Behnam Zamanzad1, Akram Sedaghat2, Masih Hosseini1, Najmeh Shahinfard1, Hedayatollah Shirzad2*
1Medical Plant Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
2Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Implication for health policy/practice/research/medical education:
Royal jelley has beneficial effect on burn healing with better wound healing effects when is used daily compared to every two days usage.
Please cite this paper as: Shirzad M, Yousofi M, Zamanzad B, Sedaghat A, Hosseini M, Shahinfard N, et al. Effects of royal jelly on sterile skin cut repair. J HerbMed Pharmacol. 2014; 3(2): 97-100.
Introduction: Following injury, inflammatory response occurs and the cells below the dermis begin to increase collagen production, then, the epithelial tissue is regenerated. Royal jelly (RJ) has anti-inflammatory activity hence, the aim of this study was to examine the effect of RJ on the induction of sterile skin incision in Balb/C mice.
Methods: In an experimental study 60 female Balb/C mice (8 weeks old) were anaesthetized with ether and a longitudinal para vertebral full thickness incision of 10 mm long was made. The animals were divided into six equal groups. Group 1 was considered as negative control. Group 2 (positive control) was treated topically with Nitrofurazon ointment, group 3 with RJ (200 mg/kg) every day, group 4 with RJ (200 mg/kg) every two days, group 5 with RJ (300 mg/kg) every day and group 6 with RJ (300 mg/kg) every two days. The wound length was measured with vernier capilar every two days up to full healing occurred and compared in different groups.
Results: There was significant difference between groups 1 or 2 and other groups (p<0.05). RJ promoted wound healing activity significantly in group 3, 5 compared to negative and positive control groups. There was no significant difference between the uses of 200 mg/kg and 300 mg/kg RJ (p>0.05).
Conclusion: The results of this study indicate that daily application of RJ possesses betters wound healing effects than Nitrofurazon and every two days usage of RJ.