دراسات عن العسل المصري و رويال جيليSTUDIES ON EGYPTIAN HONEY AND ROYAL JELLY
دراسات عن العسل المصري و رويال جيلي
STUDIES ON EGYPTIAN HONEY AND ROYAL JELLY
SAMIR YOUSEF ABDELSAID YOUSEF
نحل العسل تنتج سبعة أنواع من الإنتاج:
1 – عسل.
2 – الشمع.
3 – هلام الملكي.
4- حبوب اللقاح (خبز النحل).
5 – دنج.
6 – يرقات النحل.
7 – سم النحل.
وتعتبر هذه المنتجات بمثابة الغذاء والدواء.
وكانت هذه الدراسة للعسل، هلام الملكي وسم النحل، في هذا
دراسة تم حفظها السم النحل والهلام الملكي عن طريق ليوفليزاتيون
ثم حلها في العسل ليكون مكملا غذائيا (1mg
من السم النحل مجفف بالتجميد +500 مل من مجفف هلام الملكي مجفف +25
مل العسل) لذلك، أجريت هذه الدراسة من أجل
التحقيق في تأثير وجود المكملات الغذائية شفويا و
لدراسة تأثيره على الأمراض التالية:
1 – مرض السكري.
2 – فقر الدم (فقر الدم الضخم الأرومات).
3- السرطان.
4 – مرض الكبد المزمن (كلد).
5. التهاب المفاصل الروماتويدي (R.A).
هدفت هذه الدراسة إلى النقاط التالية:
1- التقييم الفيزيائي والكيميائي والميكروبيولوجي والحسي
من عسل النحل عينات من البرسيم والحمضيات والقطن والنبق.
2- دراسة التركيب الكيميائي للهلام الملكي بعد و
قبل ليوفيليزاشيون.
3- دراسة الرخاء الكيميائي والفسيولوجي و
حساب الجرعة المميتة LD50 من السم النحل.
4- دراسة تأثير تغذية المواليد الجدد المجففة بالهلام الملكي
والتأثير على أوزانها وأطوالها.
5- التجارب البيولوجية على الفئران.
6- دراسة استخدام المكملات الغذائية و
تأثير علاجي على بعض الأمراض البشرية مثل السرطان، المزمنة
وأمراض الكبد (كلد)، وفقر الدم، والتهاب المفاصل الروماتويدي (R.A) و
داء السكري.
الجزء (1):
1. الفيزيائية مميزة، الميكروبيولوجية و
التقييم الحسي لعينات النحل العسل.
1.1 الخصائص الفيزيائية لعينة نحل العسل:
تراوحت عينات ف نحل العسل بين 3.89-4.23 ل
الحمضيات العسل والعسل النبق على التوالي، و محددة
جاذبية عينات نحل العسل التي تراوحت بين 1.42 –
1.59 للحمضيات، النبق والبرسيم عينات نحل العسل
على التوالي. بركس عينات نحل العسل تراوحت بين
78.99 -80.17 لعسل باكثورن وعسل الحمضيات على التوالي.
تراوحت الأحماض الكلية (عينات حمض الجلوكونيك) بين
4.20 – 4.40 لعسل الحمضيات وعسل البخور على التوالي.
1.2 لزوجة عينات عسل النحل:
عسل القطن أعرض أعلى اللزوجة والنبق
العسل، البرسيم العسل الثاني في التوالي، اللزوجة في
عينات النحل العسل يتناسب عكسيا مع درجة الحرارة.
1.3 التجمد نقطة الاكتئاب، نسبة الرطوبة من النحل
عينات العسل:
تجميد نقطة الاكتئاب من عينات العسل النحل للعسل
حل (15٪) تراوحت بين -1.53 درجة مئوية إلى -1.22Cº ل
عسل النبق وعسل القطن على التوالي، للعسل
(68٪) تراوحت بين -5.82Cº إلى -5.43Cº ل
عسل النبق والعسل البرسيم على التوالي. رطوبة
تراوحت نسبة عسل النحل بين 17.22-
19.16 لعسل القطن وعسل الحمضيات على التوالي.
1.4 الخصائص الكيميائية لعينات النحل العسل:
وتراوح محتوى السكريات الكلية لعينات نحل العسل
بين 78.39 – 80.72 لعسل الحمضيات والعسل البرسيم
على التوالي. وتراوح محتوى البروتين من عينات نحل العسل
بين 0.23-0.27 لعسل الحمضيات والعسل البرسيم
على التوالي.
الجزء 2):
2. تأثير ليوفليزاتيون على التركيب الكيميائي للملكية
هلام.
2-1 تأثير التجويف على الهلام الملكي:
هذه الدراسة تعترف بأن محتوى الرطوبة من هلام الملكي
تراوحت بين 64.17 – 68.15 قبل ليوفليزاتيون، بعد
تراوحت الرطوبة ليوفليزاتيون بين 3.41 – 5.62. بروتين
وتراوح محتوى هلام الملكي بين 25.90 – 27.44 بعد
ليوفليزاتيون، قبل ليوفليزاتيون البروتين كان 8.62- 9.14.
(هاد) 10- تراوح حمض الهيدروكسيل -2 ديكونويك بين 2.71 –
2.91 قبل ليوفليزاتيون، بعد ليوفليزاتيون كان 8.13 – 8.70.
2.2 المحتوى المعدني من هلام الملكي:
المحتوى المعدني من هلام الملكي من البوتاسيوم، الكالسيوم،
المغنيسيوم، الزنك، الحديد، النحاس كان (600.0، 60.0، 59.0، 0.90،
6.0، 0.30) مغ / 100 g.
2.3 محتوى فيتامين من هلام الملكي:
محتوى الفيتامينات من هلام الملكي من النياسين (B3)،
البيريدوكسين (B6)، الثيامين (B1)، الريبوفلافين (B2) وبانتوثينيك
كان حمض (12.20، 3.10، 1.30، 1.50 و 3.20) ملغ / 100G.
2.4 محتوى الهرمونات من هلام الملكي:
محتوى الهرمونات من هلام الملكي من التيستوستيرون،
بروجسترون، برولاكتين واستراديول كان (0.20، 4.61، 70.80 و
52.03) (الشامات / 100 جرام).
الجزء (3): –
3. التركيب الكيميائي، والازدهار الفسيولوجية و
الجرعة المميتة LD50 من السم النحل.
3.1 إنتاج سم النحل لمدة عام واحد من السم
مستعمرة (ملغ) / يوم ومحتوى رطوبة (طازجة و
بعد ليوفيليزاتيون):
وكان أعلى إنتاج سم النحل من مستعمرة واحدة في
سبتمبر، ليصل إلى 310mg / يوم، في حين أن أدنى إنتاج
كان في يناير تصل إلى 210mg / يوم.
3-2 التركيب الكيميائي لسم النحل الطازج والمجفف بالليوف:
كان محتوى الرطوبة من السم النحل 84 – 90٪ من قبل
ليوفليزاتيون وكان 5 – 8٪ بعد ليوفليزاتيون، و
تراوحت الفوسفورية من 1.20 – 1.50٪، وأصبحت 9.60
– 11.20٪ بعد ليوفليزاتيون، تراوحت هيالورونيداز من
0.28 – 0.32٪ بعد ليوفليزاتيون أصبح 2.0 – 2.20٪. على ال
وغيرها من ناحية المحتوى مجفف بالتجميد السم ميليتي
SUMMARY Honey bee produces seven types of productions: 1 – Honey. 2 – Wax. 3 – Royal jelly. 4 – Pollen (bee bread). 5 – Propolis. 6 – Bee larva. 7 – Bee venom. These products are considered to be as food and medicine. This study was for honey, royal jelly and bee venom, in this study we were saved bee venom and royal jelly by lyophlization then dissolved them in honey to have a dietary supplement (1mg of lyophilized bee venom +500 ml of lyophilized royal jelly +25 ml honey) Therefore, this study was conducted in order to investigate the effect of having the dietary supplement orally and to study its effect on the following diseases: 1 – Diabetes. 2 – Anemia(megaloblastic anemia). 3 – Cancer. 4 – Chronic liver disease (CLD). 5. Rheumatoid arthritis (R.A). This study aimed at the following points: 1 – Physical, chemical, Microbiological and Sensory evaluation of bee honey samples of Clover, Citrus, Cotton and Buckthorn. 2 – Study the Chemical composition of royal jelly after and before lyophilization. 3 – The study of the chemical, physiological prosperities and calculate the lethal dose LD50 of bee venom. 4 – Study the effect of feeding newborns lyophlized royal jelly and the impact on weights and lengths of them. 5 – Biological experiments on rats. 6 – Study of the using of the dietary supplement and its therapeutic effect on some human disease like cancer, chronic liver disease (CLD), anemia, rheumatoid arthritis (R.A) and diabetes. Part (1): 1. Physicochemical characteristic, microbiological and sensory evaluation of honey bee samples. 1.1 Physical characteristics of honey bee sample: PH of honey bee samples was ranged between 3.89-4.23 for Citrus honey and Buckthorn honey respectively, the Specific gravity of honey bee samples which was ranged between 1.42 – 1.59 for Citrus, Buckthorn and Clover honey bee samples respectively. Brix of honey bee samples was ranged between 78.99 -80.17 for Buckthorn honey and Citrus honey respectively. The total acids as (gluconic acid) samples was ranged between 4.20 – 4.40 for citrus honey and Buckthorn honey respectively. 1.2 Viscosity of bee honey samples: Cotton honey exhibited the highest viscosity and Buckthorn honey, Clover honey was second in respectively, the viscosity in honey bee samples inversely proportional with temperature. 1.3 Freezing point depression, moisture percentage of bee honey samples: Freezing point depression of honey bee samples for honey solution (15%) was ranged between -1.53 Cº to -1.22Cº for Buckthorn honey and Cotton honey respectively, for honey solution (68%) was ranged between -5.82Cº to -5.43Cº for Buckthorn honey and Clover honey respectively. Moisture percentage of bee honey samples was ranged between 17.12- 19.16 for Cotton honey and Citrus honey respectively. 1.4 Chemical characteristics of bee honey samples: Total sugars content of bee honey samples was ranged between 78.39 – 80.72 for Citrus honey and Clover honey respectively. Protein content of honey bee samples was ranged between 0.23-0.27 for Citrus honey and Clover honey respectively. Part (2): 2. Effect of lyophlization on chemical composition of royal jelly. 2.1 The effect of lyophlization on royal jelly: This study recognize that the moisture content of royal jelly ranged between 64.17 – 68.15 before lyophlization, after lyophlization moisture was ranged between 3.41 – 5.62. Protein content of royal jelly ranged between 25.90 – 27.44 after lyophlization, before lyophlization the protein was 8.62- 9.14. (HAD) 10- hydroxyl-2deconoic acid was ranged between 2.71 – 2.91 before lyophlization, after lyophlization it was 8.13 – 8.70. 2.2 Mineral content of royal jelly: The mineral content of royal jelly of Potassium, Calcium, Magnesium, ZinK, Iron, Copper was (600.0, 60.0, 59.0, 0.90, 6.0, 0.30) mg/100 g. 2.3 Vitamin content of royal jelly: The vitamins content of royal jelly of Niacin (B3), Pyridoxine (B6), Thiamin (B1), Riboflavin (B2) and Pantothenic acid was ( 12.20, 3.10, 1.30, 1.50 and 3.20) mg/100g. 2.4 Hormones content of royal jelly: The hormones content of royal jelly of Testosterone, Progesterone, Prolactine and Estradiol was (0.20, 4.61, 70.80 and 52.03) (moles/100g). Part (3):- 3. Chemical composition, physiological prosperities and lethal dose LD50 of bee venom. 3.1 The production of bee venom for one year from one colony (mg)/ day and its moisture content (fresh and after lyophilization): The highest production of bee venom from one colony was in September, reaching to 310mg /day, while the lowest production was in January reaching to 210mg / day. 3.2 Chemical composition of fresh and lyophlized bee venom: The moisture content of bee venom was 84 – 90 % before lyophlization and it was 5 – 8 % after lyophlization, the phospholipids was ranged from 1.20 – 1.50% and became 9.60 – 11.20% after lyophlization, hyaluronidase was ranged from 0.28 – 0.32% after lyophlization it became 2.0 – 2.20% . On the other hand lyophilized bee venom content of melitine was ranged between 52.0 – 56.0%. Ash content bee venom was 0.06 % before lyophlization and after lyophlization it was 0.46 while a total sugar was 0.31% before lyophlization and after lyophlization it was 1.90. 3.3 The physiological composition of lyophlized bee venom: Physiological composition of lyophilized bee venom samples for Histamine % was ranged between 0.75 – 0.80 on April and January respectively. Dopamine% was ranged between 0.20 – 0.25 on January and December respectively. Noradrenalin% was ranged between 0.08 – 0.10 on April, October and January respectively. 3.4 Mean lethal dose L.D50 of the bee venom after subcutaneous injection in adult and weanling male and female: LD50 for adult’s male and female rats was (1 – 6) mg/kg of body weight; the same result was for weanling male and female rats. Part (4): 4. Effect of feeding with lyophlized royal jelly on infants. 4.1 The effect of feeding non breast feeding on infants (formula feeding) using artificial milk +300 mg lyophilized Royal Jelly on (lengths and weight): Weight of infants who had formula feeding of using artificial milk with 300mg of lyophilized royal jelly was increased from 2642.50 g (the new born weight) to 8503.50 g at 12 months and their length was increased from 52.75cm at the new born to 79.0cm at the 12 months, beside that these infants were preterm and was born at 7 months, so there curves of weights and lengths is similar to infants that born on 9 months and feeding breast feeding only. The most important thing that infants who had formula feeding did not have any antibiotics as a treatment all the period of having that formula. Part (5): 5. Biological experiments of rats. 5.1 Effect of treatment with 1g( 1mg of lyophilized bee venom +500 mg of lyophilized royal jelly + 25 ml honey)/kg of body weight on some diseases. The use of bee venom and royal jelly improved the therapeutic efficiency of some diseases: improving the sexual efficiency, the functions of the kidney and decrease the rate of glucose by using the doses of 500 mg and 1 gram of (1mg of lyophilized bee venom +500mg of lyophilized royal jelly +25mg honey) / kg of body weight orally daily for adult male rats for a month. This study showed that the treatment of adult male rats by 1 gram (1mg of lyophilized bee venom +500mg of lyophilized royal jelly +25mg honey) / kg of body weight orally alone resulted in increasing the weights of the testis and the body of the epididymus, sperm count, testosterone hormone, the percentage of live sperm, and glutathione level, accompanied with decrease in malondialdehyde level and the percentage of sperm abnormality. The oral administration of (1mg of lyophilized bee venom +500mg of lyophilized royal jelly +25mg honey) in doses of 500 mg and 1 g/kg body weight today for one month increased urea concentration in serum and decreased in urine, urea clearance in urine was also decreased as compared to control group. (1mg of lyophilized bee venom +500mg of lyophilized royal jelly +25mg honey) in a dose of 1 g/kg body weight daily decreased serum, urine and creatinine levels. Decrease in serum glucose level was also observed when the test animals were treated with (1mg of lyophilized bee venom +500mg of lyophilized royal jelly +25mg honey) in a dose of 500mg and 1g /kg body weight daily. Remarkable for one month was showed increasing in body weight of adult rats that treated with 1g (1mg of lyophilized bee venom +500mg of lyophilized royal jelly +25mg honey) /kg of body weight compared with control group. Part (6): 6. Utilization of the dietary supplement and its therapeutic effect on some human disease like cancer, chronic liver disease (CLD), anemia, rheumatoid arthritis (R.A) and diabetes. 6.1 Effect of using of the dietary supplement of 1g( 1mg of lyophilized bee venom +500 mg of lyophilized royal jelly + 25 ml honey)/kg of body weight and its therapeutic effect on Diabetes mellitus (DM) patients: Effect of treat patients with 1g (1 mg lyophilized bee venom + 500mg of lyophlized royal jelly + 25 ml honey)/kg of body weight orally to diabetes mellitus (DM) patient was decrease in HbA1c%from 8.29% before the treatment to 6.22% after the treatment. 6.2 Effect of using of the dietary supplement of 1g( 1mg of lyophilized bee venom +500 mg of lyophilized royal jelly + 25 ml honey)/kg of body weight and its therapeutic effect on Anemia patients: Effect of treat patients with 1g (1 mg lyophilized bee venom + 500mg of lyophlized royal jelly + 25 ml honey)/kg of body weight orally to Anemia patients there was increase in Hb%from 8.16% before the treatment to 10.32% after the treatment. 6.3 Effect of using of the dietary supplement of 1g( 1mg of lyophilized bee venom +500 mg of lyophilized royal jelly + 25 ml honey)/kg of body weight and its therapeutic effect on Rheumatoid arthritis (R.A) patients: Effect of treat patients with 1g (1 mg lyophilized bee venom + 500mg of lyophlized royal jelly + 25 ml honey)/kg of body weight orally to Rheumatoid arthritis (R.A) patients that for Clinical assessment , Joint swelling & mobility , Morning st.fitnessthere, Analgesia and Satisfaction the results was (76.7%) improved, ESR was 69.33 before the treatment which decreased to 46.67 after the treatment and Rheumatoid factor was 53.00 before the treatment which decreased to 36.40. 6.4 Effect of using of the dietary supplement of 1g( 1mg of lyophilized bee venom +500 mg of lyophilized royal jelly + 25 ml honey)/kg of body weight and its therapeutic effect on Cancer patients: Effect of treat patients with 1g (1 mg lyophilized bee venom + 500mg of lyophilized royal jelly + 25 ml honey)/kg of body weight orally to Cancer patients, the result was 35% improved, HB% before the treatment was 9.35 which was decreased and become 7.53 after the treatment while the Tumor marker (CEA) was 94.55 before the treatment which decrease a little amount by 0.05 and became 94.50 after the treatment. 6.5 Effect of using of the dietary supplement of 1g( 1mg of lyophilized bee venom +500 mg of lyophilized royal jelly + 25 ml honey)/kg of body weight and its therapeutic effect on Chronic Liver Disease (CLD) patients: Effect of treat patients with 1g (1 mg lyophilized bee venom + 500mg of lyophilized royal jelly + 25 ml honey)/kg of body weight orally to Chornic liver disease (CLD) patients 60% improved, Brilirbin before the treatment was 2.97 which was decreased and become 2.04 after the treatment while the PT was 17.82 before the treatment which decreased and become 15.60, while the SGPT was 70.40 before the treatment which decreased and become 50.08 after the treatment, Albumin was 2.55 before the treatment which increased by 0.33 and become 2.88 after the treatment, Finally PCR million was 2.09 before the treatment which decreased and become 1.10 after the treatment.
ملخص